論文種別	その他
言語種別
表題	Identification and distribution of tryptophan hydroxylase (TPH)-positive neurons in the planarian Dugesia japonica.
掲載誌名	正式名：Neuroscience research 略　 称：Neurosci Res
掲載区分	国外
巻・号・頁	59(1),101-6頁
著者・共著者	Nishimura Kaneyasu, Kitamura Yoshihisa, Inoue Takeshi, Umesono Yoshihiko, Yoshimoto Kanji, Takeuchi Kosei, Taniguchi Takashi, Agata Kiyokazu
発行年月	2007/09
概要	We identified a full-length tryptophan hydroxylase (TPH) gene of planarian Dugesia japonica from a head EST database, and named it DjTPH. Based on whole-mount in situ hybridization and immunofluorescence analyses, DjTPH mRNA and protein were mainly expressed in the nervous system, especially ventral nerve cords and eye pigment cells. Furthermore, DjTPH immunoreactivity was clearly detected at commissure axonal connections in the ventral nerve cords. 5-HT was significantly decreased in DjTPH-knockdown planarians compared with control animals. These results suggest that DjTPH is required for 5-HT biosynthesis, and DjTPH antibody is a useful marker for serotonergic neurons in planarians.
NAID	17624455