| 論文種別 | 原著論文 |
| 言語種別 | 英語 |
| 表題 | Activation of Nuclear Factor-kappa B in Endothelial Cells of Chronic Subdural Hematoma Outer Membranes. |
| 掲載誌名 | 正式名：Neurosurgery 略 称：Neurosurgery ISSNコード：15244040/0148396X |
| 掲載区分 | 国外 |
| 巻・号・頁 | 80(4),pp.571-578 |
| 著者・共著者 | Osuka Koji, Watanabe Yasuo, Usuda Nobuteru, Aoyama Masahiro, Kawaguchi Reo, Takeuchi Mikinobu, Takayasu Masakazu |
| 発行年月 | 2017/04 |
| 概要 | BACKGROUND:Chronic subdural hematoma (CSDH) is considered an angiogenic and inflammatory disease. Nuclear factor-kappa B (NF-κB) induces the production of inflammatory cytokines and adhesion molecules, which play an essential role in angiogenesis and inflammation. Recently, the double-stranded RNA-activated protein kinase (PKR) was shown to directly interact with NF-κB subunits to influence its transcriptional activity.OBJECTIVE:To examine the expression of NF-κB signaling pathway components and PKR in CSDH outer membranes.METHODS:Eight patients whose outer membranes were successfully obtained during trepanation surgery were included in this study. The IκBα, IKKβ, IKKγ, NF-κB, phosphorylated ( p )-NF-κB, and PKR expression levels were examined using western blotting analysis. NF-κB expression was also examined using immunohistochemistry. We investigated whether CSDH fluid could activate NF-κB in cultured endothelial cells in Vitro.RESULTS:The IκBα, IKKβ, IKKγ, and NF-κB levels were approximately the same. Additionally, p -NF-κB and PKR were detected at similar levels. Immunostaining showed that NF-κB was expressed in the vascular endothelium. p -NF-κB expression in endothelial cells was significantly induced immediately after treatment with CSDH fluid. Furthermore, NF-κB activation was significantly inhibited by treatment with antibodies directed against vascular endothelial growth factor.CONCLUSION:PKR might activate NF-κB through vascular endothelial growth factor in endothelial cells, which might be associated with endothelial cell proliferation in the CSDH outer membrane. Thus, the NF-κB signaling pathway could play a critical role in CSDH growth. |
| DOI | 10.1093/neuros/nyw100 |
| PMID | 28362919 |